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Dihybrid Cross Calculator

Dihybrid Cross Calculator

A, B - Dominant alleles

a, b - Recessive alleles


Mother's trait 1

Mother's trait 2

Father's trait 1

Father's trait 2


Table of Content

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Make use of this free dihybrid cross calculator if you are willing to calculate the probability of gene inheritance for your upcoming offspring. Making an addition, this dihybrid punnett square calculator 2 traits will create a chance percentage table for each gene manipulation by utilising two traits and four alleles at the same time.

Want to know how, let’s move on to the article below to know more!

What Is Dihybrid Crossover?

In the light of genetic and biological science:

“A cross among two individuals (Father and Mother) followed by a couple of observe traits that is controlled by two different genes is known as dihybrid crossover”

Possible Combinations In Dihybrid Crossover:

There exist four possible combinations of both male and female gametes in case of AaBb parent genes. These include:

  • Among all the gametes, half of them would actually get dominant alleles while the resting half would only get recessive alleles
  • As a whole, both the parents can produce a quarter of the alleles AB, Ab, aB, and ab that is almost 25% of the total

Dihybrid Punnett Square:

A punnett square is basically a table listing possible genotype combinations for the offspring. It depends upon the combinations of the genotypes of both the parents. Let’s have a look at the basic punnett square table that is given below:


Ab Ab ab ab
ab Aabb Aabb aabb



Aabb Aabb aabb aabb
ab Aabb Aabb aabb



Aabb Aabb aabb


This free dihybrid cross calculator can immediately calculate the genetic variation in terms of punnett square combinations for your offspring.

Moreover, you can also make use of the free blood type calculator if you’re looking for the punnett square combinations in the blood group of a newly born baby.

How To Do Punnett Squares With 2 Traits?

Punnett squares with 2 traits gives us 4*4 table patterns with different probabilities that are quite difficult to get a grip on.

This is because:

  • There are exactly 16 different sets of crosses you have to tackle
  • Genotype possibilities are also up to 9 that creates a confusion during prediction
  • There are total 81 dihybrid punnett square combinations that you need to cope with
  • Both the parents (father and mother) have 4 different alleles

But let us code here whatever the complicated pattern is, our dihybrid cross calculator will let you predict very fast due to its fast estimations. How does it sound to you?

Dihybrid Cross Inheritance (Example):

Let us understand the dihybrid inheritance with the following example:


Two parents are there who are about to welcome their newbie. The mother is blonde and has curly hair. While father has straight and light hair. Now following the inheritance law, predict the punnett square gene combination for the upcoming baby.

Brief Discussion:

So here we need to look for the traits exactly that are:

  • Colour of the hair
  • Type of hair

Now let us make a supposition that the colour of hair is the dominant allele (A) while the type is considered the recessive allele (B).

Being an offspring of both the parents, both colour and type of the hair will be inherited to the child. For our convenience, let us assume that:

A – curly hair (dominant)
a – straight hair (recessive)
B – dark hair (dominant)
b – light hair (recessive)

The free dihybrid cross calculator would also arrange the alleles in the same pattern but saving you a lot of your precious time.


  • The allele combination for the father will be ab. This is because father produces two identical copies of the alleles that are a, a, b, b

For the mother, there are two possible combinations:

  • A, a, when mother has two different dominant alleles and this is considered in terms of hair colour
  • b, b, when mother is homozygous and this condition holds for type of the mother hair

Worksheet for the 4*4 punnett square with 2 traits is given as follows:


Ab Ab ab ab
ab Aabb Aabb aabb



Aabb Aabb aabb aabb
ab Aabb Aabb aabb



Aabb Aabb aabb


Now from the sheet, you can better predict what are the chances for each pair of the inherited alleles in the offspring.


Phenotype refers to the traits of an individual that are easily observable. These include the eye colour, hair colour, nose shape, body shape, and others like that.


Genotypes are the genetic transformations that are transferred from parents to the new offsprings.

Genotype and Phenotype Ratios:

Here the phenotypes for a couple of traits are give as:

Aabb = Ab
aabb = ab

Now here the probabilities for both of these allele combinations is:

0.5:0.5 or 1:1

It does mean the 50% trait of the offspring would belong to the set Aabb, while the rest will belong to the set aabb. You can also verify this by utilising this free dihybrid cross calculator.

The probabilities for the punnett square combinations is given as follows:

6.25 : 12.5 : 6.25 : 12.5 : 25 : 12.5 : 6.25 : 12.5 : 6.25

Now look at the above ratios and figure out the smallest among what you have to do is to divide all the ratios by this smallest ratio:

= 6.25/6.25 : 12.5/6.25 : 6.25/6.25 : 12.5/6.25 : 25/6.25 : 12.5/6.25 : 6.25/6.25 : 12.5/6.25 : 6.25/6.25

= 1 : 2 : 1 : 2 : 4 : 2 : 1 : 2 : 1

How Dihybrid Cross Calculator Works?

This dihybrid punnett square calculator 2 traits lets you observe the traits of offspring by giving the following inputs:


  • Select traits for both parents (father and mother)
  • After you make selections, tap the calculate button


The free dihybrid calculator provides the following probability estimations:

  • Estimates the probability of dihybrids cross for each allele pair
  • Display the phenotypes and genotypes of the crossover combinations for the offspring


What is difference between Monohybrid and dihybrid cross?

In monohybrid cross, the cross happening occurs variates in one trait only within F1 generation offspring of parents. While in dihybrid cross, the trait becomes tw

What is genotypic ratio of Dihybrid?

According to the most accurate estimation as done by our punnett square generator, the genotype ratio for dihybrid is 12:3:1.

What means allele?

A particular version of DNA sequence at a specific genomic location is termed allele. And if you want to know about this genetic combination, you may be subject to the free allele frequency calculator.

Which gene is dominant?

The biological transformation relation among alleles is known as dominancy. Usually, both the parents donate a couple of gene alleles. Now if both are same, they belong to a similar family structure.but if they are different, one of them is masked and is called recessive. While the other one becomes the dominant gene.

What is difference between gene and allele?

A gene is basically a biological encoder of Deoxyribonucleic Acid (DNA). while on the other hand, an allele is just a corresponding form of gene.

What is dominance law?

The dominance law was proposed by a famous biologist Mendel that stated:

“When parents with pure, contrasting traits are crossed together, only one form of trait appears in the next generation. The hybrid offspring will exhibit only the dominant trait in the phenotype.”


Crossing over makes it possible to perform random shuffling of genetic information. And all this happens during the gamete formation in both the parents and the offspring. And due to this shuffling, new gametes are formed that are quite different and this is what makes offspring different from his/her parents and siblings. And if you want to predict the genetic allele structure for your baby, you are actually in need of utilising this dihybrid cross calculator.


From the source of Wikipedia: Punnett square, Zygosity, Monohybrid cross, Reginald Punnett

From the source of Khan Academy: Probabilities in genetics, Alleles and genes, The law of segregation, Pedigrees

From the source of Lumen Learning: Non-Mendelian Punnett Squares, Multiple Alleles, Penetrance and Expressivity